Monoclonal Anti-SAFB antibody produced in mouse
SIGMA/SAB2702149
Synonym: HAP; HET; SAF-B1; SAFB1
Product Type: Product-on-demand
Immunofluorescence Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HET/SAF-B [6F7](SAB2702149) antibody at 1:200 dilution.
Western Blotting HET/SAF-B antibody [6F7] detects HET/SAF-B protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 5% SDS-PAGE HET/SAF-B antibody [6F7] (SAB2702149) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Detection of human HET SAF-B protein in HeLa whole cell lysate using GeneTex monoclonal antibody SAB2702149
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6F7 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG1 |
| mol wt | 102 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| UniProt accession no. | Q15424  |
| Application: | For ICC/IF: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 2 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. |
| Biochem/physiol Actions: | Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| Immunogen: | A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |