Monoclonal Anti-COPS5 antibody produced in mouse
SIGMA/SAB2702152
Synonym: CSN5; JAB1; MOV-34; SGN5
Product Type: Product-on-demand
Western Blotting Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody (SAB2702152) diluted at a dilution of 1:500.
Western Blotting Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE SAB2702152 diluted at 1:10,000
Western Blotting Sample (50 μg of whole cell lysate) A: Rat liver 10% SDS PAGE SAB2702152 diluted at 1:1000
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2A10 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG2b |
| mol wt | 38 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, rat, mouse, Drosophila |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:500-1:3000 | |
| UniProt accession no. | Q92905  |
| Application: | For IP: Use at a concentration of 0.5 - 1 μg/mL. For WB: Use at a dilution of 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. |
| Biochem/physiol Actions: | JAB-1 is a 38 kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the ""Signalosome"", which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | The full-length mouse JAB-1 gene expressed in E. coli. |
| Physical form: | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |