Monoclonal Anti-THOC1 antibody produced in mouse
SIGMA/SAB2702154
Synonym: HPR1; P84; P84N5
Product Type: Product-on-demand
Immunofluorescence p84 antibody [5E10] detects p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p84 protein stained by p84 antibody [5E10] (SAB2702154) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Western Blotting Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE SAB2702154 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: HeLa B: HeLa nucleus 7.5% SDS PAGE SAB2702154 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7.5% SDS PAGE SAB2702154 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (50 μg of whole cell lysate) A: mouse Cerebellum 7.5% SDS PAGE SAB2702154 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (SAB2702154) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Western Blotting p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5% SDS-PAGE p84 antibody [5E10] (SAB2702154) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (SAB2702154) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (SAB2702154) diluted at 1 : 1000.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5E10 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG2b |
| mol wt | 84 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, hamster, monkey, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | Q96FV9  |
| Application: | Recommended Starting Dilutions: For ICC/IF: Use at a concentration of 0.5 - 2 μg/mL. For IP: 1:100-1:500. For WB: Use at a concentration of 0.3 - 2 μg/mL. For ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. |
| Biochem/physiol Actions: | P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Amino acids 15-374 of human p84 expressed in E. coli. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |