Monoclonal Anti-RPA3 antibody produced in mouse
SIGMA/SAB2702158
Synonym: REPA3
Product Type: Product-on-demand
Immunohistochemistry RPA14 antibody [11.1] detects RPA14 protein at nucleus on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. RPA14 antibody [11.1] (SAB2702158) dilution: 1:200.
Western Blotting Sample (30 μg of whole cell lysate) A: MCF7 15% SDS PAGE SAB2702158 diluted at 1:500
Western Blotting RPA14 antibody [11.1] detects RPA14 protein by Western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg MCF-7 whole cell lysate/extract 15% SDS-PAGE RPA14 antibody [11.1] (SAB2702158) dilution: 1:1000
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 11.1 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG1 |
| mol wt | 14 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1000 |
| immunoprecipitation (IP): suitable | |
| western blot: 0.1-2 μg/mL | |
| UniProt accession no. | P35244  |
| Application: | For IP: Use at a concentration of 0.1 - 2 μg/mL. For WB: Use at a concentration of 0.1 - 2 μg/mL. For IHC-P: 1:100-1:1000. Predicted molecular weight: 14 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Biochem/physiol Actions: | Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Full-length human RPA-14 expressed in E. coli.. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |