Monoclonal Anti-TSG101 antibody produced in mouse
SIGMA/SAB2702167
Synonym: TSG10; TSG10; VPS23
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using TSG101(SAB2702167) antibody at 1:100 dilution.
Western Blotting Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (SAB2702167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (SAB2702167) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (SAB2702167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blotting Sample (30 μg of whole cell lysate) A: HeLa 10% SDS PAGE SAB2702167 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (SAB2702167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate
Western Blotting TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (SAB2702167) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (SAB2702167) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Sample (30 μg of whole cell lysate) A: HeLa 10% SDS PAGE SAB2702167 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A10 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG1 |
| mol wt | 46 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, monkey, mouse, rat, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: suitable | |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:500-1:3000 | |
| UniProt accession no. | Q99816  |
| Application: | Suggested starting dilutions are as follows: WB: 1:500-1:3000. Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-TSG101 antibody has been used in western blotting and immunoprecipitation (IP) analyses. |
| Biochem/physiol Actions: | The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus. |
| Biochem/physiol Actions: | Tumor susceptibility gene 101 (TSG101) is essential for the generation of central supramolecular activation cluster, and the signal termination of T cell receptor (TCR) microclusters (MC). It plays an important role in TCR downregulation and separation of TCR- major histocompatibility complex (MHC)-peptide from signaling complexes, that are enriched with protein kinase C θ (PKCθ). TSG101-4A10 recognizes TSG101 protein, the product of a recently identified tumor susceptibility gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Tumor susceptibility gene 101 (TSG101) is an endosomal sorting complex required for transport (ESCRT) component, present on endosomal membranes. This gene is located on chromosome 11p15. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus. |
| Immunogen: | Amino acids 167-374 of TSG101 protein expressed in E. coli. |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |