Monoclonal Anti-RBBP8 antibody produced in mouse
SIGMA/SAB2702168
Synonym: JWDS; RIM; SAE2; SCKL2
Product Type: Product-on-demand
Immunofluorescence CtIP antibody [19E8] detects CtIP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CtIP protein stained by CtIP antibody [19E8] (SAB2702168) diluted at 1:100. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 19E8 |
| monoclonal | |
| concentration | 1.3 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG |
| mol wt | 125 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunocytochemistry: 1:100-1:1000 | |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| proximity ligation assay: suitable | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q99708  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: Assay-dependent dilution, WB: 1:500-1:1000 (3% Milk/TBST overnight), ChIP assay: Assay-dependent dilution, PLA: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disruppted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Human CtIP |
| Physical form: | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |