Monoclonal Anti-XPC antibody produced in mouse
SIGMA/SAB2702172
Synonym: RAD4; XP3; XPCC
Product Type: Product-on-demand
Immunofluorescence XPC antibody [3.26] detects XPC protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] (SAB2702172) diluted at 1:100. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Western Blotting XPC antibody [3.26] detects XPC protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] (SAB2702172) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Western blot image of human XPC from HeLa whole cell lysate (40 μg/lane) detected using rabbit polyclonal (lanes 1 and 2, catalog numbers SAB2702177 (1:500) and SAB270217, respectively) or monoclonal (lane 3, catalog number SAB2702172) antibodies.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3.26 |
| monoclonal | |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | liquid |
| isotype | IgG1 |
| mol wt | 120-125 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q01831  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant XPC purified from E. coli |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |