Monoclonal Anti-Urease B antibody produced in mouse
SIGMA/SAB2702182 - clone GT962, affinity isolated antibody
Product Type: Product-on-demand
Western Blotting Sample (0.1 μg of whole cell lysate) A: Helicobacter pylori 7.5% SDS PAGE SAB2702182 diluted at 1:10,000
ELISA ELISA detection of Helicobacter pylori urease B using SAB2702182 for capture. Coating Ab: mouse anti-ureB(SAB2702182, 100 ng/well ) Sample: H. pylori. Lysate (OD600 = 0.08~0.1, 5x dilute from above) Capture Ab: rabbit anti-ureB 2-Ab: goat anti-rabbit IgG-HRP (1:1000)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT962, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 62kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | Helicobacter pylori |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 5000-20000 |
| Application: | Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Helicobacter pylori Urease B. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |