Monoclonal Anti-HA tag antibody produced in mouse
SIGMA/SAB2702196 - clone GT423, affinity isolated antibody
Synonym: Anti-HA Antibody; HA Tag Detection Antibody; Mouse Anti-HA Tag
Product Type: Product-on-demand
Immunofluorescence HA tag antibody [GT423] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA-recombinant protein stained by HA tag antibody [GT423] (SAB2702196) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (SAB2702196) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting HA Tag antibody [GT423] detects HA Tag protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg whole cell lysate/extract of HA-tagged protein expressing HeLa cells 7.5% SDS-PAGE HA Tag antibody [GT423] (SAB2702196) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments. IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells A. Control with 2 μg of preimmune mouse IgG B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (SAB2702196) 5% SDS-PAGE The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (SAB2702132) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT423, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1000-10000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:1000-1:10000. Not yet tested in other Application Longs. Optimal working dilutions should be determined experimentally by the end user.Monoclonal Anti-HA tag antibody produced in mouse has been used in western blot analysis. |
| Biochem/physiol Actions: | Hemagglutinin (HA) is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The hemagglutinin (HA) gene is mapped to segment 4 of Influenza B viral genome. HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. Influenza hemagglutinin protein is a nona-peptide derived from the major spike membrane glycoprotein of the human influenza virus. This strain specific glycoprotein is a homotrimer of 84kDa monomers. |
| Immunogen: | The immunogen used to generate this antibody corresponds to HA tag |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |