Monoclonal Anti-RFP antibody produced in mouse
SIGMA/SAB2702202 - clone GT1610, affinity isolated antibody
Synonym: Mouse Anti-Red Fluorescent Protein
Product Type: Product-on-demand
Immunofluorescence RFP-tag antibody detects RFP-recombinant protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP-recombinant protein stained by RFP-tag antibody (SAB2702202) diluted at 1:2000. Red: RFP is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
Western Blotting RFP antibody [GT1610] detects RFP (red fluorescent protein ) protein by western blot analysis. A. 30μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of RFP-transfected 293T cells 12% SDS-PAGE RFP antibody [GT1610] (SAB2702202) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (SAB2702202) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (SAB2702202) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT1610, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:500-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |