Monoclonal Anti-Lamin B2 antibody produced in mouse
SIGMA/SAB2702205 - clone GT144, affinity isolated antibody
Synonym: LAMB2; LMN2
Product Type: Product-on-demand
Immunohistochemistry Lamin B2 antibody [GT144] detects LMNB2 protein at nucleus membrane on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Lamin B2 antibody [GT144] (SAB2702205) dilution: 1:200.
Immunofluorescence Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (SAB2702205) diluted at 1:1000. Green: alpha Tubulin antibody. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Western Blotting Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (SAB2702205) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 μg HepG2 whole cell lysate/extract B. 30 μg HepG2 cytosol lysate/extract C. 30 μg HepG2 nuclear lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] (SAB2702205) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Lamin B2 antibody [GT144] detects LMNB2 protein by western blot analysis. A. 30 μg U87-MG whole cell lysate/extract B. 30 μg SK-N-SH whole cell lysate/extract C. 30 μg IMR32 whole cell lysate/extract D. 30 μg SK-N-AS whole cell lysate/extract 7.5% SDS-PAGE Lamin B2 antibody [GT144] (SAB2702205) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation Lamin B2 antibody [GT144] immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 μg of Lamin B2 antibody [GT144] (SAB2702205) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [GT144] (SAB2702205) diluted at 1 : 1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT144, monoclonal |
| concentration | 0.81mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 68kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q03252  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Lamin B2. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |