Monoclonal Anti-Cofilin 1 antibody produced in mouse
SIGMA/SAB2702206 - clone GT567, affinity isolated antibody
Synonym: CFL; HEL-S-15; cofilin
Product Type: Product-on-demand
Immunohistochemistry Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Cofilin 1 antibody [GT567] (SAB2702206) dilution: 1:200.
Immunofluorescence Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (SAB2702206) diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Western Blotting Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (SAB2702206) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 12% SDS-PAGE Cofilin 1 antibody [GT567] (SAB2702206) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (SAB2702206) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (SAB2702206) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT567, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 19kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect immunofluorescence: suitable | |
| western blot: suitable | |
| UniProt accession no. | P23528  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Cofilin 1. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |