Monoclonal Anti-RFP antibody produced in mouse
SIGMA/SAB2702214 - clone GT1433, affinity isolated antibody
Synonym: Mouse Anti-Ref Fluorescent protein
Product Type: Product-on-demand
Western Blotting RFP antibody detects RFP protein by Western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg 293T whole cell extract expressing GFP-tagged protein C. 30 μg 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGE RFP antibody (SAB2702214) dilution: 1:10,000
Immunoprecipitation RFP tag antibody immunoprecipitates RFP-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of RFP-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of RFP-fused protein by 2 μg of RFP tag antibody (SAB2702214) 12% SDS-PAGE The immunoprecipitated RFP-fused protein was detected by RFP tag antibody (SAB2702214) diluted at 1:1000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT1433, monoclonal |
| concentration | 0.98mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 27kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 5000-20000 |
| Application: | Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-RFP antibody produced in mouse has been used in chromatin immunoprecipitation (ChIP) assay. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Red fluorescent protein (RFP) is obtained from the Discosoma coral. It can be used as a fluorescent probe for optical microscopy and acts as a biological marker for biomolecule imaging. RFPs exhibit fluorescence emission beyond 57 0nm. They have lower light-scattering, low photo-toxicity and less autofluorescence interference of cells. RFPs are extremely useful for whole body and deep tissue imaging. |
| Immunogen: | Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |