Monoclonal Anti-6X His tag antibody produced in mouse
SIGMA/SAB2702218 - clone GT359, affinity isolated antibody
Synonym: Mouse Anti-Hexa Histidine tag
Product Type: Product-on-demand
Immunofluorescence 6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] (SAB2702218) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting 6x His antibody detects 6x His protein by Western blot analysis. A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins 15% SDS-PAGE 6x His antibody (SAB2702218) dilution: 1:5000
Western Blotting 6x His antibody detects 6x His protein by Western blot analysis. A. 2 μg GST-recombinant protein B. 2 μg His-recombinant protein C. 1 μg His-recombinant protein D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein E. 0.1 μg His-recombinant protein E. 0.1 μg His-recombinant protein 12 % SDS-PAGE 6x His antibody (SAB2702218) dilution: 1:5000
Western Blotting 6x His antibody detects 6x His protein by Western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12 % SDS-PAGE 6x His antibody (SAB2702218) dilution: 1:5000
Immunoprecipitation 6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments. IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells A. 1/20 lysate/extract of transfected HeLa cells B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] (SAB2702218) 12% SDS-PAGE The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] (SAB2702218) diluted at 1:1000.
ELISA 6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] (SAB2702218).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT359, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting. |
| Biochem/physiol Actions: | 6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | 6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein. |
| Immunogen: | The immunogen used to generate this antibody corresponds to 6X His tag |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |