Monoclonal Anti-6X His tag antibody produced in mouse
SIGMA/SAB2702219 - clone GT359, affinity isolated antibody
Product Type: Product-on-demand
Western Blotting 6x His antibody conjugated HRP detects 6x His protein by western blot analysis. IPTG-induced (+, ) cell lysate from E. coli expressing different GST-tagged proteins and His-tagged proteins (2 μl) were separated by 12% SDS-PAGE, and the membrane was blotted with 6x His antibody conjugated HRP (SAB2702218-01) at a dilution of 1:20,000.
Western Blotting 6X His tag antibody [GT359] (HRP) detects 6xHis Tag protein by Western blot analysis. A. 5 μl non-induced cell lysate from E. coli expressing His-tagged proteins B. 5 μl IPTG-induced cell lysate from E. coli expressing His-tagged proteins 15% SDS-PAGE 6X His tag antibody [GT359] (HRP) (SAB2702218-01) dilution: 1:5000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT359, monoclonal |
| concentration | 0.4mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| isotype | IgG3 |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | western blot: 1:1000-1:20000 |
| Application: | Suggested starting dilutions are as follows: WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | 6X His tag does not affect the structure and function of the protein and can be used in antibody production. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | 6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein. |
| Immunogen: | The immunogen used to generate this antibody corresponds to 6X His tag |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | 0.05M Tris, 0.15M NaCl (pH7.4). 0.025% ProClin 300 was added as a preservative. |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H317 - H411 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |