Monoclonal Anti-6X His tag antibody produced in mouse
SIGMA/SAB2702220 - clone GT161, affinity isolated antibody
Synonym: Anti-Hexa histidine tag
Product Type: Product-on-demand
Western Blotting 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT161] (SAB2702220) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. 2 μg GST-recombinant protein B. 2 μg His-recombinant protein C. 1 μg His-recombinant protein D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT161] (SAB2702220) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting 6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT161] (SAB2702220) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
ELISA 6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT161] (SAB2702218).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT161, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| western blot: suitable |
| Application: | Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | The immunogen used to generate this antibody corresponds to 6X His tag |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |