Monoclonal Anti-Dengue virus Envelope protein antibody produced in mouse
SIGMA/SAB2702233 - clone GT643, affinity isolated antibody
Product Type: Product-on-demand
Immunofluorescence Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde. Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (SAB2702233) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde. Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (SAB2702233) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis. A. 30 μg BHK21 whole cell lysate/extract B. 30 μg whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 12% SDS-PAGE Envelop protein (Dengue virus 2) antibody [GT643] (SAB2702233) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT643, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | 51-60kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | Dengue virus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: 1:100-1:2,000 | |
| western blot: 1:500-1:3,000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:2000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Dengue virus is an arthropod borne flavivirus. Dengue virus (DENV) is a human pathogen, known to cause one of the major illness, dengue fever (DF) in humans. DENV belongs to the Flaviviridae family. The virus attaches and enters the host cell with the help of a major envelope protein glycoprotein, E. The dengue envelope protein sequence consists of two putative glycosaminoglycan-binding motifs at the carboxy terminus. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |