Monoclonal Anti-GEF-H1 antibody produced in mouse
SIGMA/SAB2702237 - clone GT336, affinity isolated antibody
Synonym: GEF; GEF-H1; GEFH1; LFP40
Product Type: Product-on-demand
Immunofluorescence GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (SAB2702237) diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Immunofluorescence GEF-H1 antibody [GT336] detects GEF-H1 protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [GT336] (SAB2702237) diluted at 1:50.
Western Blotting GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] (SAB2702237) dilution: 1:500
Western Blotting GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [GT336] (SAB2702237) dilution: 1:500
Western Blotting GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg C8D30 whole cell lysate/extract B. 30 μg BCL-1 whole cell lysate/extract C. 30 μg Raw264.7 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [GT336] (SAB2702237) dilution: 1:500
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT336, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 500-3000 | |
| UniProt accession no. | Q92974  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:50-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |