Monoclonal Anti-Dengue virus Type 2 NS5 protein antibody produced in mouse
SIGMA/SAB2702242 - clone GT361, affinity isolated antibody
Product Type: Product-on-demand
Immunofluorescence NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by NS5 (Dengue virus 2) antibody [GT361] (SAB2702242) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde. Green: NS5 (Dengue virus 2) protein stained by NS5 (Dengue virus 2) antibody [GT361] (SAB2702242) diluted at 1:2000. Blue: Hoechst 33342 staining.
Western Blotting NS5 (Dengue Virus 2) antibody [GT361] detects NS5 protein by western blot analysis. A. 30 μg BHK21 whole cell lysate/extract B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 10% SDS-PAGE NS5 (Dengue Virus 2) antibody [GT361] (SAB2702242) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells 7.5% SDS-PAGE NS5 (Dengue virus 2) antibody [GT361] (SAB2702242) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT361, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 103kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | Dengue virus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1000-10000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:2000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus Type 2 NS5 protein. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |