Monoclonal Anti-5-Methylcytosine / 5-mC antibody produced in mouse
SIGMA/SAB2702243 - clone GT4111, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) dilution: 1:50.
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) dilution: 1:200.
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) diluted at 1:200.
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis. Sample: 24 hours-post-fertilization zebrafish embryo. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) dilution: 1:50.
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) dilution: 1:200.
Immunohistochemistry 5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-Methylcytosine / 5-mC antibody [GT4111] (SAB2702243) dilution: 1:200.
Dot Blotting SAB2702243 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls. DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (SAB2702243) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
Immunoprecipitation SAB2702243 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (SAB2702243) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT4111, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunohistochemistry: suitable | |
| methylated DNA immunoprecipitation (MeDIP): suitable |
| Application: | Suggested starting dilutions are as follows: Dot: Assay-dependent dilution, EM: Assay-dependent dilution, IHC-P: 1:100-1:1000, IHC-Wm: 1:50-1:500, MeDIP: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |