Monoclonal Anti-Factor X antibody produced in mouse
SIGMA/SAB2702247 - clone GT268, affinity isolated antibody
Synonym: FX; FXA
Product Type: Product-on-demand
Western Blotting Factor X antibody [GT268] detects Factor X protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human F10-transfected 293T cells 10 % SDS-PAGE Factor X antibody [GT268] (SAB2702247) dilution: 1:10,000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT268, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | 55kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:100-1:1,000 |
| western blot: 1:5,000-1:20,000 | |
| UniProt accession no. | P00742  |
| Application: | Suggested starting dilutions are as follows: WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Factor X. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |