Monoclonal Anti-DDDDK tag antibody produced in mouse
SIGMA/SAB2702252 - clone GT231, affinity isolated antibody
Product Type: Product-on-demand
Western Blotting DDDDK tag antibody [GT231] (SAB2702252) detects DDDDK-tagged proteins by western blot. A. Untransfected 293T whole cell lysate/extract (30 μg ) B.-D. Whole cell lysate/extracts (30 μg) from transfected 293T cells expressing different DDDDK-tagged proteins. 10% SDS-PAGE DDDDK tag antibody [GT231] (SAB2702252) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments. IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells. A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] (SAB2702252) 7.5% SDS-PAGE The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] (SAB2702252) diluted at 1 : 5000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT231, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1000-10000 |
| Application: | Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | The immunogen used to generate this antibody corresponds to DDDDK tag |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |