Monoclonal Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody produced in mouse
SIGMA/SAB2702266 - clone GT223, affinity isolated antibody
Product Type: Product-on-demand
Immunofluorescence Influenza A Virus H1N1 Hemagglutinin (HA) antibody [GT223] detects Influenza A Virus H1N1 Hemagglutinin (HA) protein by immunofluorescent analysis. Sample: DF1 cells infected with Influenza A virus (WSN) . Green: Influenza A Virus H1N1 Hemagglutinin (HA) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody [GT223] (SAB2702266) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting Influenza A Virus H1N1 Hemagglutinin (HA) antibody [GT223] detects Influenza A Virus H1N1 Hemagglutinin (HA) protein by Western blot analysis. A. 5 μg DF1 whole cell lysate/extract B. 5 μg whole cell lysate/extract of Influenza A (WSN) infected DF1 cells 7.5 % SDS-PAGE Influenza A Virus H1N1 Hemagglutinin (HA) antibody [GT223] (SAB2702266) dilution: 1:1000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT223, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | influenza A virus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 500-3000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in HA of the H3 subtype play a key role in host range restriction and receptor specificity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Influenza virus hemagglutinin (HA) precursor is encoded by the gene mapped on segment 4. The encoded protein is a 76kDa surface glycoprotein and it exists as trimers on the envelope. |
| Immunogen: | Recombinant fragment corresponding to C-terminal region of Influenza A virus Hemagglutinin (HA) (A/WSN/1933(H1N1)). |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |