Monoclonal Anti-O-GlcNAc transferase antibody produced in mouse
SIGMA/SAB2702272 - clone GT678, affinity isolated antibody
Synonym: HRNT1; O-GLCNAC
Product Type: Product-on-demand
Immunofluorescence O-GlcNAc transferase antibody [GT678] detects O-GlcNAc transferase protein at nucleus (mainly) and cytoplasm (less) by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody [GT678] (SAB2702272) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 50 μg mouse brain lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] (SAB2702272) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting O-GlcNAc transferase antibody [GT6G8] detects O-GlcNAc transferase protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 7.5% SDS-PAGE O-GlcNAc transferase antibody [GT6G8] (SAB2702272) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT678, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 500-3000 | |
| UniProt accession no. | O15294  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human O-GlcNAc transferase. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |