Monoclonal Anti-PRMT1 antibody produced in mouse
SIGMA/SAB2702289 - clone GT779, affinity isolated antibody
Synonym: ANM1; HCP1; HRMT1L2; IR1B4
Product Type: Product-on-demand
Immunofluorescence PRMT1 antibody [GT779] detects PRMT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT1 protein stained by PRMT1 antibody [GT779] (SAB2702289) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blotting PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10% SDS-PAGE PRMT1 antibody [GT779] (SAB2702289) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blotting PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg NIH-3T3 whole cell lysate/extract D. 30 μg Raw264.7 whole cell lysate/extract E. 30 μg C2C12 whole cell lysate/extract 10% SDS-PAGE PRMT1 antibody [GT779] (SAB2702289) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT779, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 500-1000 | |
| UniProt accession no. | Q99873  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRMT1. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |