Monoclonal Anti-mCherry antibody produced in mouse
SIGMA/SAB2702291 - clone GT857, affinity isolated antibody
Synonym: Anti Mcherry Antibody; Anti mCherry Antibody - Monoclonal Anti-mCherry antibody produced in mouse
Product Type: Product-on-demand
Western Blotting mCherry antibody [GT857] detects mCherry protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5% SDS-PAGE mCherry antibody [GT857] (SAB2702291) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT857, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: 1:500-1:3,000 |
| Application: | Suggested starting dilutions are as follows: IHC: Assay-dependent dilution, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | The acceptor (mCherry) possess high photostability and shows resistance to photobleaching. It acts as a good FRET (fluorescence resonance energy transfer) acceptor. mCherry helps in real-time analysis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The acceptor (mCherry) is a monomeric protein obtained from stony-coral DsRed. It is expressed in the cytoplasm. |
| Immunogen: | Full length mCherry recombinant protein |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |