Monoclonal Anti-Histone H4K20me2 (dimethyl Lys20) antibody produced in mouse
SIGMA/SAB2702306 - clone GT1851, affinity isolated antibody
Synonym: H4FA
Product Type: Product-on-demand
Immunofluorescence Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (SAB2702306) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blotting Histone H4 (di methyl Lys20) antibody [GT1851] detects Histone H4 (di methyl Lys20) protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [GT1851] (SAB2702306) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (SAB2702306). Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (SAB2702306).
ChIP Assay Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT1851, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 11kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | dimethylation (Lys20) |
| technique(s) | ChIP: suitable |
| dot blot: suitable | |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: 1:1,000-1:10,000 |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:1000-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me2 (dimethyl Lys20). |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |