Monoclonal Anti-SOD2 antibody produced in mouse
SIGMA/SAB2702309 - clone GT582, affinity isolated antibody
Synonym: IPOB; MNSOD; MVCD6
Product Type: Product-on-demand
Western Blotting Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT582] (SAB2702309) diluted at 1:10,000.
Western Blotting SOD2 antibody [GT582] detects SOD2 protein by western blot analysis. A. 50 μg mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [GT582] (SAB2702309) dilution: 1:5000
Western Blotting SOD2 antibody [GT582] detects SOD2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract | D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [GT582] (SAB2702309) dilution: 1:5000
Western Blotting SOD2 antibody [GT582] detects SOD2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [GT582] (SAB2702309) dilution: 1:5000
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT582, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 25kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1000-1:10000 |
| UniProt accession no. | P04179  |
| Application: | Suggested starting dilutions are as follows: WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SOD2. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |