Monoclonal Anti-HIF2 alpha antibody produced in mouse
SIGMA/SAB2702342 - clone GT125, affinity isolated antibody
Synonym: ECYT4; HIF2A; HLF; MOP2
Product Type: Product-on-demand
Immunofluorescence HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (SAB2702342) diluted at 1:100. Red: Actin filaments were labeled with Phalloidin.
Immunofluorescence HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (SAB2702342) diluted at 1:100. Blue: Hoechst 33342 staining.
Immunofluorescence HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Samples: PC-12 cells mock (left) and treated with 500 μM CoCl2 for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (SAB2702342) diluted at 1:100. Blue: Hoechst 33342 staining.
Western Blotting HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (SAB2702342) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunoprecipitation Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody [GT125] (SAB2702342). Western blot analysis was performed using HIF2 alpha antibody [GT125] (SAB2702342).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT125, monoclonal |
| concentration | 3mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 96kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | Q99814  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:700, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Hypoxia-inducible factor 2-α (HIF2) regulates oxygen physiology and modulates the hypoxic response. It favors chondrosarcoma progression. Mutations in the HIF2 Phe-540 residue abolishes its interaction with von hippel lindau (VHL) and prolyl hydroxylase domain-2 (PHD2). HIF2 regulates erythropoietin synthesis and is implicated in the pathophysiology of excessive erythrocytosis (EE). Mutations in the HIF2 gene impair response to the changes in oxygen tension. A gain-of-function mutation of HIF2 results in Zhuang syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Hypoxia-inducible factor 2-α (HIF2) also known as endothelial PAS domain protein 1 (EPAS1) belongs to the helix-loop-helix/ Per-Arnt-Sim (PAS) transcription factor family. The HIF2 gene is mapped to human chromosome 2p21. HIF2 is expressed in endothelial cells, hepatocytes, lung, cardiomyocytes, and renal fibroblasts. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |