Monoclonal Anti-TEF1 antibody produced in mouse
SIGMA/SAB2702354 - clone GT13112, affinity isolated antibody
Synonym: AA; NTEF-1; REF1; TCF-13
Product Type: Product-on-demand
Immunofluorescence TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] (SAB2702354) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Western Blotting TEF1 antibody [GT13112] detects TEF1 protein by western blot analysis. whole cell extracts and extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (SAB2702354) at a dilution of 1:500.
Immunoprecipitation Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (SAB2702354). Western blot analysis was performed using TEF1 antibody [GT13112] (SAB2702354).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT13112, monoclonal |
| concentration | 2.66mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 48kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P28347  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |