Monoclonal Anti-Filamin B antibody produced in mouse
SIGMA/SAB2702362 - clone GT1372, affinity isolated antibody
Synonym: ABP-278; ABP-280; AOI; FH1
Product Type: Product-on-demand
Immunohistochemistry Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Filamin B antibody [GT1372] (SAB2702362) diluted at 1:250.
Immunohistochemistry Filamin B antibody [GT1372] detects Filamin B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Filamin B antibody [GT1372] (SAB2702362) diluted at 1:250.
Western Blotting Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT1372] (SAB2702362) diluted at 1:500.
Immunoprecipitation Immunoprecipitation of Filamin B protein from HeLa whole cell extracts using 5 μg of Filamin B antibody (SAB2702362). Western blot analysis was performed using Filamin B antibody (SAB2702362). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT1372, monoclonal |
| concentration | 1.92mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 278kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | O75369  |
| Application: | Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |