Monoclonal Anti-Iba1 antibody produced in mouse
SIGMA/SAB2702364 - clone GT10312, affinity isolated antibody
Synonym: AIF-1; IBA1; IRT-1; IRT1
Product Type: Product-on-demand
Immunohistochemistry Iba1 antibody [GT10312] detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody [GT10312] (SAB2702364) diluted at 1:200.
Immunohistochemistry Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse hippocampus. Green: Iba1 protein stained by Iba1 antibody [GT10312] (SAB2702364) diluted at 1:250. Blue: Fluoroshield with DAPI.
Immunohistochemistry Iba1 antibody [GT10312] detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 antibody [GT10312] (SAB2702364) diluted at 1:200.
Immunofluorescence Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody [GT10312] (SAB2702364) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blotting Iba1 antibody detects Iba1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (SAB2702364) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GT10312, monoclonal |
| concentration | 2.03mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 17kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:1000 |
| indirect immunofluorescence: suitable | |
| western blot: 500-3000 | |
| UniProt accession no. | P55008  |
| Application: | Monoclonal Anti-Iba1 antibody produced in mouse has been used: • in the double immunofluorescence • in the immunofluorescence • in immunohistochemistry |
| Biochem/physiol Actions: | Ionized calcium-binding adapter molecule 1 (IBA1) interacts with filamentous actin (F‐actin) and L‐fimbrin. Its expression is modulated during injury. Inflammatory cytokines also induce IBA1 expression. IBA1 expression is implicated in the graft rejection in coronary artery vasculopathy (CAV). It is highly expressed in hepatocellular carcinoma and may serve a potential marker and therapeutic target. Increased expression of AIF1 in synovial fluid and peripheral blood monocytes is implicated in rheumatoid arthritis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Ionized calcium-binding adapter molecule 1 (IBA1), also called the allograft inflammatory factor 1 (AIF1) is mapped to human chromosome 6p21.33. It encodes a 17 kDa protein and comprises calcium binding EF hand motif. IBA1 is expressed in lung, tonsil, lymph node and spleen. In addition,it is also expressed in macrophages and glial cells. |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Iba1. |
| Other Notes: | Purification: Affinity purified by Protein G |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |