Monoclonal Anti-HEC1 antibody produced in mouse
SIGMA/SAB2702380 - clone 9G3.23, affinity isolated antibody
Synonym: HEC; HEC1; HsHec1; KNTC2
Product Type: Product-on-demand
Immunofluorescence Confocal immunofluorescence staining (Olympus FV10i) of HEC1, a kinetochore outer layer marker. U2OS cells were fixed by 4% PFA and costained with Hec1 9G3.23 AB (Red; SAB2702380; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green)), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Immunofluorescence Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
Immunofluorescence Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 protein stained by Hec1 antibody [9G3.23] (SAB2702380) diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody. Blue: Hoechst 33342 staining.
Western Blotting HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (SAB2702380) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate
Western Blotting Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (SAB2702380) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 9G3.23, monoclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | 80kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | hamster, kangaroo rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunofluorescence: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| proximity ligation assay: suitable | |
| western blot: suitable | |
| UniProt accession no. | O14777  |
| Application: | Suggested starting dilutions are as follows: FACS: Assay-dependent dilution, ICC/IF: 1:100-1:1000, IHC: Assay-dependent dilution, IP: Assay-dependent dilution, WB: 1:500-1:3000, PLA: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Human HEC1 protein consisting of amino acids 56-642. |
| Other Notes: | Purification: Protein G Affinity Purified |
| Physical form: | Phosphate-buffered saline, no preservative added. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |