Anti-ARID2 antibody produced in rabbit
SIGMA/SAB2702507 - affinity isolated antibody
Synonym: BAF200; p200
Product Type: Product-on-demand
Immunofluorescence ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ARID2 protein stained by ARID2 antibody (SAB2702507) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Western Blotting ARID2 antibody detects ARID2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human ARID2-transfected 293T cells 5 % SDS-PAGE ARID2 antibody (SAB2702507) dilution: 1:5000
Western Blotting ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (SAB2702507) diluted at 1:3000.
Immunoprecipitation Immunoprecipitation of ARID2 protein from HepG2 whole cell extracts using 5 μg of ARID2 antibody (SAB2702507). Western blot analysis was performed using ARID2 antibody (SAB2702507). EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation ARID2 antibody immunoprecipitates ARID2 protein in IP experiments. IP samples: HeLa whole cell extract A. 35 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (SAB2702507) D. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (SAB2702508) 5 % SDS-PAGE The immunoprecipitated ARID2 protein was detected by ARID2 antibody (SAB2702507) diluted at 1:3000. [EasyBlot anti-Rabbit IgG was used as a secondary reagent].
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 197kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 500-10000 | |
| UniProt accession no. | Q68CP9  |
| Application: | Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Biochem/physiol Actions: | ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM] |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. |
| Other Notes: | Purification: Purified by antigen-affinity chromatography |
| Physical form: | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H317 - H411 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |