Anti-RSPO1 antibody produced in rabbit
SIGMA/SAB3500046 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CRISTIN3; Anti-R-spondin1; Anti-RSPO
Product Type: Chemical
Immunohistochemistry Anti-RSPO1: Cat. No. SAB3500046: RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/mL.
Immunoblotting Anti-RSPO1: Cat. No. SAB3500046: RSPO1 in rat spleen tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001033722   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q2MKA7 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry-froz |
| Biochem/physiol Actions: | RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | R-spondin 1 (RSPO1) is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular β-catenin, thereby regulating functions mediated by β-catenin such as cell fate decisions and embryonic patterning. It possesses two furin-like cysteine-rich domains at the amino-terminus, a thrombospondin domain and a carboxy-terminal tail. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members. The gene encoding RSPO1 is localized on human chromosome 1p34.3. |
| Immunogen: | RSPO1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human RSPO1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500046 . |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |