Anti-AFAP1L2 antibody produced in rabbit
SIGMA/SAB3500054 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Actin filament associated protein 1-like 2; Anti-XB130
Product Type: Chemical
Immunohistochemistry Anti-AFAP1L2: Cat. No. SAB3500054: AFAP1L2 in human liver tissue with AFAP1L2 antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 μg/mL.
Immunoblotting Anti-AFAP1L2: Cat. No. SAB3500054: AFAP1L2 in mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_001001936   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q8N4X5 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3b, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist. |
| Immunogen: | AFAP1L2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human AFAP1L2. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500054 . |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |