Anti-Seasonal H1N1 Hemagglutinin antibody produced in rabbit
SIGMA/SAB3500060 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Common flu H1; Anti-Flu H1; Anti-HA; Anti-Seasonal Influenza A (H1N1) Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
ELISA Anti-Seasonal H1N1 Hemagglutinin: Cat. No. SAB3500060: Anti-Seasonal Hemagglutinin antibody at 2 μg/mL specifically recognizes seasonal influenza virus A H1N1 but not swine-origin influenza virus (S-OIV) A H1N1 Hemagglutinin protein.
ELISA Anti-Seasonal H1N1 Hemagglutinin: Cat. No. SAB3500060: Anti-Seasonal H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.
Immunoblotting Anti-Seasonal H1N1 Hemagglutinin: Cat. No. SAB3500060: Hemaggutinin using recombinant seasonal Hemagglutinin (lanes A and B) and swine-origin Hemagglutinin (lanes C and D) with anti-seasonal Hemagglutinin antibody (5235) at 2 μg/mL (lanes A and C) and anti-swine-origin Hemagglutinin antibody (5237) at 2 μg/mL (lanes B and D).
ELISA ELISA results using Seasonal H1N1 Hemagglutinin antibody at 1 μg/mL and the blocking and corresponding peptides at 50, 10, 2 and 0 ng/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | predicted mol wt 62 kDa |
| NCBI accession no. | ACA28844   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | viral |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: suitable | |
| UniProt accession no. | B0VX44 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States (2). The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic (3). Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin (4). The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups (5). This antibody is specific for the seasonal H1N1 influenza Hemagglutinin and will not recognize the corresponding Hemagglutinin sequence from the swine-origin H1N1 influenza (A/California/14/2009 (H1N1)). |
| Immunogen: | Hemagglutinin antibody was raised against a synthetic peptide from the Hemagglutinin protein. The peptide sequence is unique from the peptide sequence for product SAB3500058 and SAB3500062. This antibody is a cognate pair with antibody SAB3500061. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500060 . |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |