Anti-NOTUM antibody produced in rabbit
SIGMA/SAB3500082 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-NOTUM pectinacetylesterase homolog
Product Type: Chemical
Immunohistochemistry Anti-NOTUM: Cat. No. SAB3500082: NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.
Immunoblotting Anti-NOTUM: Cat. No. SAB3500082: NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_848588   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q6P988 |
| Application: | Anti-NOTUM antibody produced in rabbit has been used in immunostaining. |
| Biochem/physiol Actions: | Notum is involved in the progression of colorectal cancer and its expression levels is higher in metastatic cells. Notum is one of the pain associated genes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by β-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway. |
| General description: | NOTUM encodes palmitoleoyl-protein carboxylesterase. In human chromosome, the gene NOTUM is localized on 17q25.3. |
| Immunogen: | NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of human NOTUM. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500082 . |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |