Anti-FRMPD4 antibody produced in rabbit
SIGMA/SAB3500116 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FERM and PDZ domain-containing 4; Anti-PDZD10; Anti-PDZK10; Anti-Preso
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of FRMPD4 in human brain tissue with FRMPD4 antibody at 5 μg/mL.
Immunofluorescence Immunofluorescence of FRMPD4 in human brain tissue with FRMPD4 antibody at 20 μg/mL.
Immunoblotting Anti-FRMPD4: Cat. No. SAB3500116: FRMPD4 in SK-N-SH cell lysate with FRMPD4 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q12767  |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD4, also known as Preso, also contains another protein interaction domain termed WW (domain with two conserved Trp residues) at its amino terminus. It was identified through a yeast two-hybrid screen using the PDZ domain of PSD-95 as bait and is highly expressed in multiple regions of the brain and is enriched in the postsynaptic density (PSD) fractions. Overexpression of FRMPD4 in cultured hippocampal neurons significantly increased the linear density of dendritic spines without changing their length and width; conversely, knockdown experiments using RNAi caused a decrease in spine density, indicating FRMPD4 positively regulates dendritic spine density but not morphology. The decreased level of FRMPD4 also resulted in reduced levels of excitatory synaptic transmission, suggesting that FRMPD4 is required for maintenance of excitatory synaptic transmission. |
| Immunogen: | FRMPD4 antibody was raised against an 18 amino acid peptide from near the center of human FRMPD4. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500116 . |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |