Monoclonal Anti-ORAI1 antibody produced in mouse
SIGMA/SAB3500127 - clone 6D11A11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Calcium release-activated calcium channel protein 1; Anti-TMEM142A; Anti-Transmembrane protein 142A
Product Type: Chemical
Immunohistochemistry Anti-Monoclonal ORAI1: Cat. No. SAB3500127: ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of ORAI1 in rat spleen tissue with ORAI1 antibody at 5 μg/mL.
Immunoblotting Anti-Monoclonal ORAI1: Cat. No. SAB3500127: ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6D11A11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | predicted mol wt 33 kDa |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q96D31  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3. |
| Immunogen: | raised against a 16 amino acid peptide from near the carboxy terminus of human ORAI1. |
| Physical form: | Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |