Anti-ADAM10 antibody produced in rabbit
SIGMA/SAB3500181 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-KUZ
MDL Number: MFCD03096586
Product Type: Chemical
Immunocytochemistry Anti-ADAM10: Cat. No. SAB3500181: Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/mL.
Immunofluorescence Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 μg/mL.
Immunoblotting Anti-ADAM10: Cat. No. SAB3500181: Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | predicted mol wt 85 kDa |
| NCBI accession no. | NP_001101   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | O14672 |
| Biochem/physiol Actions: | ADAM metallopeptidase domain 10 (ADAM10) activates Notch proteins and has a role in embryonic development. It functions as a molecular scissor and cleaves the extracellular domains of proteins. The protein is a therapeutic target for a variety of diseases and malignancies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ADAM metallopeptidase domain 10 or A disintegrin and metalloprotease 10 (ADAM10) is a transmembrane protein which is widely distributed. It consists of an amino-terminal signal sequence, a prodomain, a metalloprotease domain, a disintegrin domain, a cysteine-rich region, a transmembrane region and a cytoplasmic tail. The gene encoding this protein is localized on human chromosome 15q21.3. |
| Immunogen: | ADAM10 antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4). |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500181 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |