Anti-AGTR1 antibody produced in rabbit
SIGMA/SAB3500209 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AT1; Anti-AT1R; Anti-Ang II; Anti-Angiotensin II receptor type 1
MDL Number: MFCD01321727
Product Type: Chemical
Immunohistochemistry Anti-AGTR1: Cat. No. SAB3500209: Immunohistochemistry of AGTR1 in mouse kidney tissue with AGTR1 antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of AGTR1 in Mouse Kidney cells with AGTR1 antibody at 20 μg/mL.
Immunoblotting Anti-AGTR1: Cat. No. SAB3500209: Western blot analysis of AGTR1 in mouse kidney tissue lysate with AGTR1 antibody at (A) 1 and (B) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | EAW78909   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P30556 |
| Application: | Anti-AGTR1 antibody produced in rabbit has been used in in situ proximity ligation assay (PLA) and immunoblotting. |
| Application: | Rabbit polyclonal anti-AGTR1 is used to tag angiotensin II receptor for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of angiotensin II receptor in the regulation of processes that affect vascular functions such as blood pressure and blood flow. |
| Biochem/physiol Actions: | Angiotensin II receptor, type 1 (AGTR1) has a key function in vascular smooth muscle contraction and maintenance of vasopressor effects by controlling aldosterone secretion. The binding of angiotensin II to AGTR1 contributes to the proliferation of vascular smooth muscle cells by the activation of G protein. The binding also results in the activation of Ca2+ dependent kinases as IP3 (inositol triphosphate) and MAPK (mitogen-activated protein kinases) for the synthesis of proteins and expression of proto-oncogenes, respectively. AGTR1 is associated with high risk of hypertension and cardiovascular diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Angiotensin II receptor, type 1 (AGTR1) is a G-protein coupled receptor possessing seven transmembrane functional domains. AGTR1 gene spanning around 45.1kb, with five exons, is located on human chromosome 3q21−q25. AGTR1 is highly expressed on endothelial cells, monocytes, podocytes, renal vascular smooth muscle cells and renal arteries. |
| General description: | Angiotensin II receptor, type 1 or AT1 receptor (AGRR1, AT1R) is a receptor for the vasoconstriction peptide angiotensin II involved in the regulation of cardiovascular effects such as blood vasoconstriction (blood pressure), aldosterone and vasopressin secretion, cardiac hypertrophy, vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, modulation of central sympathetic nervous system activity, cardiac contractility, central osmocontrol and extracellular matrix formation. |
| General description: | Rabbit polyclonal anti-AGTR1 reacts with mouse, human, and rat angiotensin II receptor. |
| Immunogen: | AGTR1 antibody was raised against a 16 amino acid peptide from near the center of human AGTR1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500209 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |