Anti-EndoG antibody produced in rabbit
SIGMA/SAB3500213 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD04040310
Product Type: Chemical
Immunohistochemistry Anti-EndoG: Cat. No. SAB3500213: Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15 μg/mL.
Immunoblotting Anti-EndoG: Cat. No. SAB3500213: Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with EndoG antibody at 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | predicted mol wt 35 kDa |
| NCBI accession no. | NP_004426   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q14249 |
| Application: | Anti-EndoG antibody produced in rabbit is suitable for immunohistochemistry, indirect ELISA and western blot analysis. |
| Biochem/physiol Actions: | ENDOG (Endonuclease G) is a mitochondrial apoptotic DNase involved in the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. During mitochondrial release and nuclear translocation of EndoG, BNIP3 interacts with the voltage-dependent anion channel (VDAC) to facilitate the process in the nucleus, it cleaves chromatin apoptotic DNA without the need of caspase activity. ENDOG causes DNA fragmentation during and after apoptosis. It is linked with cardiac hypertrophy and Parkinson′s disease. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ENDOG (Endonuclease G) is a 28kDa mitochondria-localized protein mapped to human chromosome 9q34.1. However, in presence of certain conditions it can translocate to the nucleus through the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. |
| Immunogen: | EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500213 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |