Anti-LIS1 antibody produced in rabbit
SIGMA/SAB3500302 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Lissencephaly 1; Anti-PAFAH; Anti-
MDL Number: MFCD03457563
Product Type: Chemical
Immunocytochemistry Anti-LIS1: Cat. No. SAB3500302: Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 μg/mL.
Immunofluorescence Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 μg/mL.
Immunoblotting Anti-LIS1: Cat. No. SAB3500302: Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| InChI | 1S/C21H39O7P/c1-2-3-4-5-6 |
| InChI key | ZQTAMPRZFOOEEP-AVQMFFATSA |
| NCBI accession no. | P43034   |
| Quality Level | 100 |
| shipped in | dry ice |
| SMILES string | [P](=O)(OCC(O)COC(=O)CCCC |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P43034 |
| Application: | Monoclonal Anti-LIS1 antibody produced in mouse has been used in immunofluorescence. |
| Biochem/physiol Actions: | The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3. |
| Immunogen: | LIS1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human LIS1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500302 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |