Anti-Bax antibody produced in rabbit
SIGMA/SAB3500343 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Bax
MDL Number: MFCD00282585
Product Type: Chemical
Immunocytochemistry Anti-Bax: Cat. No. SAB3500343: Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.
Immunofluorescence Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 μg/mL.
Immunoblotting Anti-Bax: Cat. No. SAB3500343: Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | AAA03619   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q07812 |
| Application: | Anti-Bax antibody produced in rabbit has been used in Western blotting. |
| Biochem/physiol Actions: | BCL2 associated X, apoptosis regulator (BAX) forms a heterodimer with B-cell lymphoma 2 (BCL2) and functions as an apoptotic activator. BAX interacts with and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in the mitochondrial membrane potential and the release of cytochrome c. The expression of BAX gene is regulated by the tumor suppressor p53 and BAX has been shown to be involved in p53-mediated apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | BCL2 associated X, apoptosis regulator (BAX) is a proapoptotic protein of the B-cell lymphoma 2 (BCL2) protein family. The gene encoding it is localized on human chromosome 19q13.33. |
| Immunogen: | Bax antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Bax. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500343 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |