Anti-PLEKHM1 antibody produced in rabbit
SIGMA/SAB3500458 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AP162; Anti-B2; Anti-OPTB6; Anti-Pleckstrin homolog domain containing family M member 1
Product Type: Chemical
Immunohistochemistry Anti-PLEKHM1: Cat. No. SAB3500458: Immunohistochemistry of PLEKHM1 in human lung tissue with PLEKHM1 antibody at 5 μg/mL.
Immunofluorescence Immunofluorescence of PLKHM1 in human lung tissue with PLKHM1 antibody at 20 μg/mL.
Immunoblotting Anti-PLEKHM1: Cat. No. SAB3500458: Western blot analysis of PLEKHM1 in human lung tissue lysate with PLEKHM1 antibody at 1 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | Q9Y4G2   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9Y4G2 |
| Biochem/physiol Actions: | PLEKHM1 (pleckstrin homology and RUN domain containing M1) is involved in the end stages of endocytic and autophagy pathways. Here, it interacts with RAB7 (member of RAS oncogene family), the HOPS (homotypic fusion and vacuole protein sorting) complex and ATG8 (autophagy-related protein 8) proteins. It plays an important role in autophagosome-lysosome fusion and endosome and autophagosome maturation. PLEKHM1 also participates in osteoclast function and bone resorption. In presence of Salmonella enterica Typhimurium, the PLEKHM1-RAB7-HOPS complex targets the Salmonella effector protein SifA (Salmonella-induced filament protein A) and controls vacuoles consisting of Salmonella. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The gene PLEKHM1 (pleckstrin homology and RUN domain containing M1) is mapped to human chromosome 17q21.31. The encoded protein is widely expressed. The protein localizes in the cytoplasm and late endosomal/lysosomal compartments. PLEKHM1 has a RH (rubicon homologous) domain, two PH (pleckstrin homology) domains and a LIR (LC3 interaction region). |
| Immunogen: | PLEKHM1 antibody was raised against an 18 amino acid peptide from near the amino terminus of human PLEKHM1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3500458 . |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |