Monoclonal Anti-EndoG antibody produced in mouse
SIGMA/SAB3500468 - clone 7G1C10, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Endonuclease G
MDL Number: MFCD04040310
Product Type: Chemical
Immunoblotting Anti-EndoG Monoclonal: Cat. No. SAB3500468: Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7G1C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | Q14249   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: suitable | |
| UniProt accession no. | Q14249 |
| Application: | Monoclonal Anti-EndoG antibody produced in mouse is suitable for indirect ELISA and western blot. |
| Biochem/physiol Actions: | ENDOG (Endonuclease G) is a mitochondrial apoptotic DNase involved in the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. During mitochondrial release and nuclear translocation of EndoG, BNIP3 interacts with the voltage-dependent anion channel (VDAC) to facilitate the process in the nucleus, it cleaves chromatin apoptotic DNA without the need of caspase activity. ENDOG causes DNA fragmentation during and after apoptosis. It is linked to cardiac hypertrophy and Parkinson′s disease. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | ENDOG (Endonuclease G) is a 28kDa mitochondria-localized protein mapped to human chromosome 9q34.1. However, in presence of certain conditions it can translocate to the nucleus through the BNIP3 (Bcl-2/adenovirus E1B 19kDa protein-interacting protein 3) cell death pathway. |
| Immunogen: | Mouse monoclonal EndoG antibody was raised against a recombinant protein corresponding to amino acids 51 – 140 of human EndoG. |
| Physical form: | Supplied in PBS with 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |