Anti-Bovine IgG (H+L), F(ab′)2 fragment-Peroxidase antibody produced in rabbit
SIGMA/SAB3700036 - affinity isolated antibody, lyophilized powder
Synonym: HRP
Product Type: Product-on-demand
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | bovine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable |
| Biochem/physiol Actions: | IgG (immunoglobulin G) antibody provides protection against bacterial, fungal and viral infections. Maternal IgG is transferred to fetus through the placenta that is vital for the immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. IgG is involved in hypersensitivity type II and type III. It helps in opsonization, complement fixation and cell-mediated cytotoxicity. |
| Biochem/physiol Actions: | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against Anti-Pepsin or Anti-Rabbit IgG F(c). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antibody format: IgG F(ab′)2 |
| General description: | IgG (immunoglobulin G) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. The variable region of IgG antibody is specific to antigens and is highly conserved. IgG antibody is abundantly present in mother′s milk. |
| Immunogen: | Bovine IgG whole molecule |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preparation Note: | Reconstitute with 500 μ;L deionized water (or equivalent). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P280 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |