Anti-Cat IgG F(ab′)2-Alkaline Phosphatase antibody produced in rabbit
SIGMA/SAB3700053 - affinity isolated antibody, buffered aqueous solution
Synonym: ALP; Alk Phos
Product Type: Product-on-demand
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg/mL |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | cat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable |
| Biochem/physiol Actions: | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Alkaline Phosphatase (calf intestine), Anti-Rabbit Serum, Cat IgG, Cat IgG F(ab’)2 and Cat Serum. No reaction was observed against Cat IgG F(c). |
| General description: | Antibody format: IgG |
| General description: | IgG (immunoglobulin G) is part of the immunoglobulin family and is widely expressed. It possesses a γ heavy chain in the constant (C) region. IgG has a monomeric 150kDa structure, with two identical heavy chains and two identical light chains. They have a molecular weight of 50kDa and 25kDa, respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains and the heavy and light chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion with papain cleaves the antibody into three fragments. Two of these fragments are identical and have antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. |
| Immunogen: | Cat IgG F(ab′)2 fragment |
| Physical form: | Supplied in 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |