Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit
SIGMA/SAB3700420 - affinity isolated antibody, lyophilized powder
Synonym: Anti-Pig IgG F(ab′)2-Peroxidase antibody produced in rabbit; Anti-Porcine IgG F(ab′)2-Peroxidase antibody produced in rabbit; HRP
Product Type: Product-on-demand
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | swine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable |
| Application: | Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).(3) |
| Biochem/physiol Actions: | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Swine IgG F(ab′)2 and Swine Serum. No reaction was seen against Swine IgG F(c) . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antibody format: IgG |
| General description: | Immunoglobulin G (IgG) is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. |
| Immunogen: | Swine IgG F(ab′)2 fragment |
| Physical form: | Supplied in 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preparation Note: | Reconstitute with 1.0 mL deionized water (or equivalent). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P280 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |